Nts which include catalase, or by non-enzymatic antioxidants which include vitamins. Catalase is believed to play a significant function within the 1st line of enzymatic antioxidant defense. It is actually a tetrameric enzyme, consisting of 4 identical subunits that include a single ferriprotoporphoryn group per subunit. Catalase reacts efficiently in peroxisomes with hydrogen peroxide (H202) to form water and molecular oxygen [10,11]. Hyperhomocysteinaemia and other cardiovascular danger aspects have already been recommended to become implicated inside the imbalance aggravation amongst pro-oxidants and antioxidants linked to its pro-oxidant properties or within the impairment of antioxidant systems. The aim of our study is always to evaluate the total antioxidant capacity and erythrocyte catalaseactivity in patients hospitalized for AMI. We aimed to access the doable relationships involving erythrocyte catalase activity, hyperhomocysteinaemia and the severity of myocardial dysfunction below ischemia reperfusion.Components and methodsStudy populationThe study consisted of 108 patients using a mean age of 63 ?12 years admitted for the division of cardiology in Farhad Hached hospital in Tunisia. The diagnostic of AMI was established in line with universal clinical criteria: chest pain which lasted for up three hours, ECG adjustments and serum troponin elevation. The handle group consisted of 81 healthy volunteers with no history of coronary artery illness, diabetes, hypertension or inflammatory illness. Their mean of age was 59 ?9 years. The electrocardiographic changes had been recorded and assessed by 2 cardiologists who had been unaware that the sufferers are integrated in our study. Coronary threat factors happen to be referred in our study population according to universal definitions. Dyslipidemia was defined when total cholesterol concentration was 5,68 mmol/l, or triglyceride concentration 2,28 mmol/l. Hyperhomocysteinaemia was defined by a raised blood total homocysteine, exceeding 15 mol/l. Informed written consent was obtained from each patient and wholesome subject based on the guidelines of our ethics’ committee.Blood collection and biochemical methodsVenous blood was collected right after overnight fasting and within 24 hours just after admission for chest pain. Serum was separated by centrifugation at 1500 g for 10 min, and then stored at -80 until the day of analysis. Serum was used for the estimation of lipid peroxidation, total homocysteine (tHcy), apolipoproteins (Apo A-1 and Apo B) levels and total antioxidant status (TAS) concentration. Total homocysteine in serum was measured by the “Abbott homocysteine assay”, a completely automated fluorescence polarization immunoassay (FPIA) from Abbott diagnostics. Serum apolipoproteins (Apo A-1 and Apo B) have been measured by immunonephelometry (Dade Behring, Marburg, Germany).Perfluoropropionic anhydride uses Plasma TAS was measured with commercial kit (Randox, Antrim, UK) in accordance with the strategy of Miller et al.41203-22-9 site [12].PMID:25147652 It could evaluate the total capacity of all antioxidants found in serum to neutralize the oxidative action of cost-free radicals. Lipid peroxidation level was estimated by measurement of thiobarbituric acid reactive substances (TBARS) in serum according to the fluorimetric system of Yagi [13]. The pink chromogen made by the reaction of thiobarbituric acid with lipid peroxidation merchandise which include malondialdehyde (MDA) was estimated making use of 1,1,3,3-tetraethoxypropaneNoichri et al. Diagnostic Pathology 2013, 8:68 http://diagnosticpathology.org/content/8/1/Page 3 ofas typical MDA. Serum TBARS levels w.